Journal: Scientific Reports

Article Title: Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy

doi: 10.1038/srep11219

Figure Lengend Snippet: After treatment, liver, white adipose tissue (WAT), muscle and blood samples were collected to prepare total cellular protein, total RNA, total lipid and serum, respectively. Expression of PCK1, G6Pase and GCK ( A ); AMPKα and pi-AMPKα ( B ); IRS1, FGF21 and PPARγ ( C , D ) protein was determined by Western blot, respectively. *: vs. control db/db mice, p < 0.05 (n = 3); Expression of FGF21 [E, *: vs. wild type mice; **: vs . control db/db mice, p < 0.05 (n = 3)], PPARγ [F, *: vs. control db/db mice, p < 0.05 (n = 3)], ATGL, HSL, CPT1α, LCAD, MCAD, ACOX1, Cyto-C, ATPase and PPARα [G, *: vs. control db/db mice, p < 0.05 (n = 3)] mRNA was determined by real time RT-PCR, respectively; Serum FGF21 levels [E, *: vs. wild type mice; **: vs . control db/db mice, p < 0.05 (n = 5)] and liver TG content [F, *: vs. wild type mice, p < 0.05 (n = 5)] were determined using assay kits, respectively.

Article Snippet: Serum samples were also used to determine FGF21 levels using a mouse FGF21 ELISA kit (Boster, Wuhan, China) according to the manufacture’s instruction.

Techniques: Expressing, Western Blot, Control, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy

doi: 10.1038/srep11219

Figure Lengend Snippet: Sequences of the primers for real time RT-PCR analysis.

Article Snippet: Serum samples were also used to determine FGF21 levels using a mouse FGF21 ELISA kit (Boster, Wuhan, China) according to the manufacture’s instruction.

Techniques: Quantitative RT-PCR

Journal: Chemico-biological interactions

Article Title: Signature of paraoxonases in the altered redox homeostasis in Alzheimer's disease.

doi: 10.1016/j.cbi.2023.110839

Figure Lengend Snippet: Fig. 4. PON1 (panel A) and PON2 (panel B) protein levels in transgenic (3xTg-AD) and non-transgenic (Non-Tg; i.e. controls) mice brain homogenate at different post-natal days (PD). Data are reported as mean ± S.E.M.; (n = 4/group). Statistical analysis was performed by 2-way ANOVA and subsequent Bonferroni post-hoc test for multiple comparisons. *p < 0.05, **p < 0.01.

Article Snippet: PON1 and PON2 proteins concentration in homogenized brains were determined by PicokineTM Mouse PON1 ELISA kit (Boster Biochem, Cambridge, MA, USA) and mouse PON2 ELISA kit (Abcam, Cambridge, UK) following the manufactured procedures.

Techniques: Transgenic Assay